| ROLE OF TP63 REARRANGEMENTS IN LYMPHOPROLIFERATIVE DISORDERS |
| Paper ID : 1006-YRC2022 |
| Authors |
|
Nada H Ahmed * Suez Canal University hospital |
| Abstract |
| Background: LPDs are a group of mature lymphocytes and NK- cell disorders that vary in clinical presentation, course and treatment. Recent molecular advances have led to updating the current classification of this group of disorders and the addition of new provisional entities. Aim of work: To assess the role of different p63 isoforms on T lymphocytes. Methods: This study was conducted in vitro on T-cell lymphoma cell lines, and on normal CD4+ T cells. We generated three plasmids that harbor the TBL1XR1/TP63 gene fusion, transduced the different cell lines by the PLVX plasmids that harbor the fusion and monitored the difference in their growth rates through MTS experiments. Results: p63 short isoform tend to have an oncogenic role in T lymphocytes and p63 long isoform tend to have a tumor suppressive effect on T lymphocytes. Conclusion: Normal CD4+ T cells is an appropriate cell model to study the function of TP63 gene fusions; TLBR3 cells could also be used when normal CD4+ T cells weren’t feasible to work with. p63 short isoforms tend to have an oncogenic effect on TLBR3 cells and p63-L tend to have a tumor suppressive effect on normal CD4+ T cells. The effect of p63-I on T cells could not be assessed. TP63 fusions could be used as an independent prognosticator in LPD patients and can have either a tumor suppressive or oncogenic role depending on the expression of its different isoforms. |
| Keywords |
| Lymphoproliferative disorders, TP63 isoforms, ΔNp63, TAp63 |
| Status: Conditional Accept (Oral Presentation) |
