| DETECTION OF LEVOFLOXACIN HEMIHYDRATE USING A HIGHLY SENSITIVE LUMINESCENT PROBE OF EU(III)-COMPLEX IN ENVIRONMENTAL SAMPLES. |
| Paper ID : 1038-YRC2022 |
| Authors |
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Rasha Hassan Mohamed Abdel all *, Zeinab Mohamed Anwar, Gasser Mohamed Khairy, Yasser Mahmoud Awad Suez Canal University |
| Abstract |
| A highly sensitive and selective spectrofluorimetric method for the rapid determination of Levofloxacin Hemihydrate antibiotic has been developed. The method is based on quenching the fluorescence of Eu(III) complex with Coumarin-3-carboxylic acid (Lc) by optimizing the luminescent complex probe concentration to 5x10-5M. The probe’s fluorescence is monitored at the Eu(III) ion’s characteristic emission wavelength of 614 nm with excitation at 350 nm. Ethanol was found to be the ideal solvent for optimum detection. Under optimal conditions linear calibration curves from 25 nmol L−1 to 100 μmol L−1 were obtained. Using the Stern-Volmer equation, the antibiotic’s detection limit (DL) in the studied complex is 3.72 µmol L-1. The effect of lag time and temperature was measured using a lag time 100 µSec. and different temperatures. Both ∆H and ∆S have positive values indicating a hydrophobic binding type. And a negative value of ∆G indicates a spontaneous reaction. Interference of heavy metal ions and other antibiotics from the same class were investigated. This method can be applied to detect antibiotic residues in a variety of environmental samples, including mineral water, tap water, and wastewater. |
| Keywords |
| Luminescent probe, Eu(III) complex, Levofloxacin hemihydrate, Fluorescence measurements, Wastewater. |
| Status: Conditional Accept (Oral Presentation) |
