| EFFECTS OF AUTOLOGOUS DENTAL PULP STEM CELLS AND MINERAL TRIOXIDE AGGREGATE ON DOGS’ DENTAL PULP |
| Paper ID : 1070-YRC2022 |
| Authors |
|
Dalia K Khalil *, Manar Selim, Rania Galhom, Mohamed El Daharawy, Hayam Hassan Suez Canal University |
| Abstract |
| This study evaluated the exposed dogs’ dental pulp response after direct capping with autologous dental pulp stem cells (DPSCs) and compared the results with MTA (Angelus, Brazil). Sixty teeth from six mongrel dogs were included (n=60), allocated into four groups; negative control (n=6), positive control (n=6), MTA (n=24), and DPSCs (n=24). DPSCs were isolated and cultured from extracted first premolars (1 premolar tooth/dog). The teeth were prepared for histological study and immunohistochemical analysis. Statistical analysis was calculated using one way and two way ANOVA tests, P ≤ 0.05. MTA induced the formation of an almost complete calcific bridge. DPSCs successfully regenerated the injured pulp and dentin. The comparison between the capping groups and time intervals had a statistically significant effect, as well as, the interaction between the two variables. DPSCs had greater ability in the reparative and regenerative process of dentin and odontoblastic differentiation by having a significantly stronger TGFβ1 expression than MTA. |
| Keywords |
| Direct pulp capping, DPSCs, MTA, TGFβ1, Dogs’ dental pulp |
| Status: Accepted (Oral Presentation) |
