| MOLECULAR CHARACTERIZATION OF VAGINAL MICROBIOTA IN HEALTHY EGYPTIAN WOMEN DURING PREGNANCY |
| Paper ID : 1071-YRC2022 (R1) |
| Authors |
|
Mariam E. Amin *, Sarah Shabayek, Marwa Azab, Khaled Ahmed Atwa, Amr Hanora Suez Canal University |
| Abstract |
| The vagina is the shelter of the normal flora which known as the “vaginal micro-biota”. These microbiota play a vital role in protection against infections caused by pathogenic bacteria, yeast, or the other organisms. Our study aimed for detection of the microbiota inhabiting the vagina during the pregnancy through using the next generation sequencing technique. A total of 36 vaginal swab samples were collected from healthy Egyptian pregnant women in Ismailia. DNA was extracted from these samples, then sequenced using Illumina MiSeq technique after PCR amplification of the V3-V4 region of 16S rRNA marker gene. Lactobacillus was the most prevalent genus during pregnancy (88%). Also, L. iners was found to be the most pervasive species in vagina during pregnancy, representing 52% of the vaginal microbiota. The protective role of these beneficial bacterial microbiota is due to secreting lactic acid which maintains the acidic lower pH (< 4.5) of the vagina. |
| Keywords |
| Vaginal microbiota, Lactobacillus iners, Illumina MiSeq, pregnancy. |
| Status: Accepted (Poster Presentation) |
