| SILDENAFIL CITRATE AND NANOFORM OF SELENIUM POTENTIATE THERAPEUTIC ANGIOGENESIS AND ELIMINATE OXIDATIVE STRESS IN DIABETIC HIND LIMB ISCHEMIA |
| Paper ID : 1080-YRC2022 |
| Authors |
|
Karim Mohamed Tawfic *, Reem Hazem, Pierre Hanna, Mona El-Azab, Yasser Moustafa Suez Canal University |
| Abstract |
| Sildenafil citrate is a highly selective inhibitor of cyclic guanine monophosphate-specific phosphodiesterase type-5. They hydrolyze specifically cGMP, and its inhibition increases cGMP levels through the activation of guanylate cyclase (GC) which in turn, activated by nitric oxide (NO) generation. leading to smooth muscle relaxation and vasodilation. Antioxidants have been reported to reduce ischemia reperfusion injury. Selenium (Se) is one of the most important antioxidants. Nanoelemental Se (SeNPs) are more biocompatible with exhibiting a dramatic decrease in toxicity. The present study investigated whether pharmacological pre-conditioning with the PDE-5 inhibitor or/and SeNPs might, through their angiogenic and antioxidant effect, respectively, reduce skeletal muscle deformation using the model of tourniquet-induced lower limb ischemia in diabetic rats. Histopathological examination revealed that treatment with combination of sildenafil and SeNPs decreased degenerative changes and inflammation in the ischemic muscle of diabetic rats. In conclusion Sildenafil and Nano selenium mediated therapeutic angiogenesis in diabetic hind limb ischemia. They improve blood supply, antioxidants delivery for cells and decrease necrosis in peripheral arterial disease (PAD). |
| Keywords |
| Sildenafil, NanoSelenium, ischemia, oxidative stress |
| Status: Accepted (Poster Presentation) |
