| Biofilm Elimination from Infected Root Canals using Four different Single Files |
| Paper ID : 1082-YRC2022 |
| Authors |
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Sarah A Hamed *1, Hayam Y Hassan2 1Suez Canal university 2Suez Canal University |
| Abstract |
| Enterococcus faecalis (E. faecalis) is the most commonly isolated bacteria from persistent periapical lesions in the form of biofilm. This study evaluated the efficacy of XP-endo Shaper, Hyflex EDM, One Curve, and Fanta. AFTM F One against E. faecalis biofilm growth inside root canals using colony forming units per millilitre (CFU mL-1) and scanning electron microscope (SEM). Eighty-five extracted mandibular premolars with straight root canals were randomly selected. After performing a pilot study (n=15) to determine the ideal incubation period for biofilm development, Sixty-five root canals were contaminated with E. faecalis and incubated for 7 days then mechanically prepared using one of the four single-files (XP-endo Shaper, Hyflex EDM, One Curve and Fanta. AFTM F One) (n=13). Five infected root canals were not instrumented as positive control group. Five non-contaminated root canals were used as negative controls. Bacterial samples were collected with sterile paper points before and after rotary instrumentation to determine the bacterial load (CFU ml-1). Root canals from each instrumentation group were evaluated topographically from the coronal, middle, and apical thirds by scanning electron microscope (SEM). Instrumentation of infected root canal with XP-endo Shaper, Hyflex DEM, and One Curve resulted in a significantly greater bacterial count reduction than those achieved with Fanta. AFTM F One with a consequent greater E. faecalis biofilm removal capacity. |
| Keywords |
| Enterococcus faecalis, Biofilm, Single file system, Scanning Electron Microscope. |
| Status: Accepted (Oral Presentation) |
